Lung cancer is a type of cancer that stems from the lungs, and is most common in individuals that smoke. Cancerous cells form when there is an altercation in the natural flow of genetic information from DNA to RNA to protein that leads to the uncontrollable growth of cells. A major focus in lung cancer research is the identification of specific mutations that may occur during this pathway that could potentially lead to the formation of cancerous cells. To seek out these mutations, comparative methods using mice models and biochemical methods such as Polymerase Chain Reaction (PCR) and Gel Electrophoresis are used. Specific genes in mouse models are genetically modified in the laboratory using biochemical techniques. Comparisons between these genetically modified mice and the non-mutated mice may give insight to the role of a particular gene in the development of lung cancer. To validate the mutation of a gene, as well as to examine the genotypes of mice models, specific genomic area containing the gene of interest is amplified using PCR, and wild type or mutant status of the target gene is determined. The combination of biochemical techniques and comparative methods using mouse models may give insight into the specific gene mutations responsible for the development of lung cancer.