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Visualization of Beadex in Adult and Larvae Brains of Drosophila melanogaster

Presenters Name: 
Cara Barmore
Co Presenters Name: 
Primary Research Mentor: 
Jay Hirsh
Secondary Research Mentor: 
Session: 
4
Location: 
Newcomb Hall Ballroom
Grant Program Recipient: 
USOAR Program
Abstract: 

Dopamine (DA) is an important neurotransmitter in the brain, and issues with DA signaling are associated with many different neurologic and psychiatric disorders. DA related processes are highly conserved, which enables the fruit fly Drosophila melanogaster to be used as a model organism to study DA. The gene Beadex (also known as Bx or Lmo) has been identified as a possible candidate for involvement in synthesis of dopamine (DA). The purpose of this research project is to visualize the expression of the gene Beadex in the brains of adult and larvae of the fruit fly Drosophila melanogaster in a specific fly line and compare it to the previously determined pattern. The fly line used in this experiment expressed both Bx and the fluorescent protein GFP. GFP exhibits fluorescence when exposed to ultraviolet light, and is used as marker for the expression of linked genes. These flies and larvae were dissected, and the brains were imaged using a confocal microscope. In the images generated, the fluorescence of GFP is shown in the locations of the brain where Bx is expressed